讲座题目：Quantal transmission at single central synapses displays large variation in size with subunit 主讲人：孙坚原 博士 主持人：曹晓华 博士 开始时间：2019-09-20 14:00:00 讲座地址：中北校区脑功能基因组学研究所一楼 主办单位：脑功能基因组学教育部重点实验室
报告人简介： 孙坚原，中国科学院“百人计划”学者，杰出青年科学基金获得者，中国科学院生物大分子科教融合卓越中心课题组长，中国科学院大学教授，神经生物与心理学教研室主任。现为中国神经科学会理事，国际学术期刊Synapse编委。主要从事突触传递与神经信息处理研究，以通讯作者在Nature，PNAS，Journal of Neuroscience等国际期刊上发表多篇论文，相关研究成果已被录入神经科学教科书《Principles of Neural Science》。他的实验室应用神经电生理、神经电化学、分子荧光成像、电子显微镜技术并结合分子生物学和生物物理学模型等方法从单个钙离子通道、单个突触囊泡、单个突触及多突触等不同层次的实验观测来研究神经信息在突触水平的量子化传递和编码的规律及其分子和细胞生物学机制。 报告内容： In the seminal work, Katz and colleagues established the quantal nature of synaptic transmission, whereby the basic unit of neurotransmission is the quantal event detected postsynaptically as a small all-or-none similar sized miniature postsynaptic potential or current (mini), in response to the neurotransmitter release from a single vesicle.However, the quantal nature of minis has never been justified by the study at single synapses, leaving the open question whether minis are identical in size and follow the principle of invariance. Here, we selectively study the quantal transmission from single active zone contained synapses using whole-cell recording and quantitative analysis. It was found that the amplitude of spontaneous and evoked miniature events from single synapses displayed large variation and were integer multiples of a subunit.This phenomenon can be observed in spontaneous mEPSCs at single synaptic boutons of early age developing calyx synapse, micrometer size Ca2+ uncaging induced quantal events at single boutons of juvenile calyx synapse and micrometer size channelrhodopsin-2 activation evoked quantal events at single boutons of mature PB-CeAL synapse.Our study suggests the large encoding scope of quantal synaptic transmission and the higher capacity of synaptic information processing than that the quantal theory implied. |
