讲座题目：A Paired-Seq Approach for High-throughput Single-cell Transcriptome Sequencing 主讲人：杨朝勇 教授 主持人：田阳 教授 开始时间：2019-07-30 14:00:00 讲座地址：闵行教师之家宾馆三楼报告厅 主办单位：华东师范大学化学与分子工程学院
报告人简介： 学士（厦门大学，1998） 硕士（厦门大学，2001） 博士（美国佛罗里达大学，2006） 博士后（美国加州大学伯克利分校, 2006-2007） 担任中国化学会化学生物学专业委员会副主任、谱学分析与仪器教育部重点实验室副主任；ACS Applied Bio Materials 副主编; BMC Biochemistry 副主编、Analytical Chemistry、Lab on a Chip、Analyst、Biomicrofluidics、Scientific Reports、Analytical and Bioanalytical Chemisry、Journal of Analysis and Testing、《分析化学》、《分析测试学报》等期刊编委/顾问编委。 先后获得美国化学会分析化学研究生奖、中国政府优秀自费留学生奖、中美化学与化学生物学教授协会杰出教授奖、福建省五四青年奖章、福建省运盛青年科技奖、福建省杰出青年基金、国家杰出青年科学基金、福建省高校领军人才、福建省科技创新领军人才、厦门市双百计划领军型创业人才、福建省自然科学优秀学术论文一等奖、中国青年分析化学家奖、中国化学会-英国皇家化学会青年化学奖、科技部中青年科技创新领军人才、中组部等9部委《全民科学素质行动计划纲要》实施工作先进个人等奖项与称号。 报告内容： Cellular heterogeneity presented by different gene expression profiles, functions and morphologies not only lies in different tissues but also even within the same cell type. Complicated heterogeneities constitute diversities of tissues, organs and systems, making them play different roles. However, once meeting with dysregulation of single cells, it can influence the whole organism, and even lead to cancers, developmental disorders and neurological disorders[1]. Single-cell transcriptome sequencing analysis provides molecular mechanisms between genetic information and proteome, which provides compressive understanding of biological processes and diseases. Traditional single-cell transcriptome sequencing methods are limited to low-throughput and high-cost[2]. The developments of microfluidic and barcoding techniques make high-throughput analysis possible but are still restricted to the loss of cellular information due to the Poisson distribution, especially when dealing with rare samples [3]. To overcome these problems, our group combined DNA barcoding technology and single cell/bead capture and pairing technology in a single microfluidic device called Paired-seq chip to realize high-throughput single-cell transcriptome sequencing. Paired-seq chip enables highly efficient and massively parallel manipulation of single cells, including capture, cell and bead pairing, separation, lysis, and mRNA capture et al. With the help of barcoding methods, the mRNAs from thousands of individual cells can be identified and counted. Thereby, a matrix of digital gene-expression measurements can be created for further analysis. This high-throughput method not only reduces the cost but also allows highly efficient capturing of rare cells. The Paired-Seq approach allows us to study cellular heterogeneity, deconstruct cell subpopulations, identify tissue origin, and decipher disease evolution mechanisms from samples with limited cell numbers such as stem cells, circulating tumor cells, and neuron cells, which are otherwise challenges to analyze with other single cell RNA sequencing platforms. |
