讲座题目：Nanofluidic Device for Cytokine Protein Analysis at Countable Molecules from Living Single B Cell 主讲人：Takehiko Kitamori 教授 主持人：田阳 教授 开始时间：2019-07-30 08:00:00 讲座地址：闵行教师之家宾馆三楼报告厅 主办单位：华东师范大学化学与分子工程学院
报告人简介： Professor Kitamori is Professor in the Department of Applied Chemistry, School of Engineering, the University of Tokyo (1998-present). He was Vice President of the University of Tokyo (2012-14), after serving as Dean of Faculty and Graduate School of Engineering. His areas of research are Micro/Extended-Nano Fluidics, Extended-Nano Space Chemistry, and Applied Laser Spectroscopy for Analytical Chemistry. He has received numerous honors for his innovative research, including CSJ Award(Chemical Society of Japan ) in 2019,Simon-Widmer Award（Swiss Chemical Society）in 2017, IBM Faculty Award in 2017 and 2008. He was awarded an Honorary Doctorate from Lund University in 2016. 报告内容： Single cell analysis is one of the most active topics of bioscience and engineering. However, almost all of the analytes are nucleic acids. Proteins are also important target for single cell proteomics, metabolomics, pathology, and so on. But the number of analytes is not only four, and absolute quantity of the analyte protein cannot be chemically amplified. Therefore, much more complicated chemical processing keeping the original sampling volume at femo liter fL ,ultrasensitive detection and determination are needed. That is the reason why single cell protein analysis requires total system integration of chemical processing at fL, and it has been still a very challenging topic. We have developed pressure driven microfluidics from the very early stage of microfluidics in the early 1990's. In the early 2000's, we established the basic concept and method of total integration of entire chemical processes for analysis, synthesis, cell experimental protocol, and so on. The key concept is micro unit operation MUO and continuous flow chemical processing CFCP. Since the same period of the early 2000's, we have pioneered nanofluidics carrying the same methodologies as microfluidics, that is, nano unit operation NUO . We applied these methodologies to total integration of the complicated chemical processing for living single cell protein analysis. An example of cytokine analysis from single B cell is introduced in the lecture. We succeeded in determining IL-6 molecules from a living single B cell which was stimulated for six hours.The device was designed by the NUO-CFCP method. Cell selection, isolation of the target single cell in a pico-liter chamber, stimulation, sampling, volumetric fractionation by a fL-pipette, sample pretreatment in a pL-flask, all unit operations of ELISA of IL-6, and DIC-TLM readout were completed in the device. We found that the B cell released four to six IL-6 molecules/min during the stimulation. This device and system will greatly contribute to elucidate the physiology of autoimmunological diseases as well as it provides novel tools for living single cell target proteomics, metabolomics, and others. |
