讲座题目：Spatio-temporal Regulation of Muscle Diversity: Insights from Omics and Genetics
主讲人：Jonathan Enriquez second Grade researcher director
主持人：杜冰 教授
开始时间：2025-04-22 13:00
讲座地址：闵行校区生科楼534小会议室
主办单位：生命科学学院
报告人简介：
Dr.Jonathan Enriquez received his PhD degree at Center of Developmental Biology, University of Toulouse 3, France. Now he is second Grade researcher director at CNRS DR2, Institute of Functional Genomics of Lyon (IGFL), Ecole Normal Supérieur of Lyon, France. He is a developmental biologist working with Drosophila. Specifically, he studies the development and maintenance of the adult locomotor system. His research focuses on three main components of this system: glial cells, motor neurons, and muscles. He uses a multiscale approach, investigating development from genes to cells, and from cells to systems and functions. His work integrates a variety of techniques and technologies, including genetics, molecular biology, confocal and 3D STED imaging, post-imaging processing, computational biology, and behavioral approaches. In recent years, he has expanded into single-cell and 3D spatial transcriptomics. Additionally,we are developing pipelines to integrate spatial transcriptomics and proteomics in 3D. In recent years, he has published a lot of papers in high impact journals such as eLife, Cell Reports and Nature Communications.
报告内容：
The wide range of muscle morphologies found across the animal kingdom enables intricate movements essential for various behaviors, such as feeding,mating, or even tasks like writing a summary for an Aurastem talk. Many animals rely on appendages for their locomotion, which feature multifiber muscles with diverse morphologies enabling unique functions. Despite extensive study and understanding of the general program of muscle development (how myoblasts produce muscles), the specific program controlling the diversity of muscle morphologies in multifiber muscles (how myoblasts produce unique muscles) remains unknown. Using Drosophila leg muscles as a model system, we have revealed that myoblasts, in parallel with the general program of myogenesis, express distinct transcriptional programs that specify muscle morphologies.Moreover,we found that the spatiotemporal progression of these programs is regulated by morphogenes secreted by the underlying epithelium. To achieve these conclusions, we used unique genetic and computational tools combined with single-cell sequencing. I would also give a couple of words of a pipeline of 3D Spatial transcriptomic(3D MERFISH) we have developed in the context of the Spatial Cell ID consortium.
